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An Independent Lab Analysis of Helping America's
Colloidal Silver/Ionic Silver Hydrosol
(Helping America is not associated nor affiliated with Natural Immunogenics in any way).
Natural Immunogenics Corp.
3265 W. McNab Rd
Pompano Beach, FL 33069Date: September 12th, 2005
Client: Helping America
Samples: H.A.
Analysis: Bacteriology
As you will see by the
independent lab (Natural Immunogenics Corp.) test results - they
clearly show that our colloidal silver/ionic silver hydrosol product
clearly out performed these top 5 brands when it came to killing the
Staphylococcus aureus "staph" hands down, none were even close!
Original - signed lab analysis on file at Helping
America.
Bacterial Strain:
Wild type Staphylococcus aureus (ATCC325952)
Methodology:
Preparation of Test Media (YT)
A. The YT media (which consists of 0.5% NaC1, 0.6% yeast extract,
0.8% tryptone and 2% agar) was dissolved in
purified (17 to 18 MegOhm) lab water and autoclaved at 121 degrees C. for 20 to 30
minutes.
B. Each 95mm sterile polystyrene culture plate (Petri Dish) was
filled to 5mm with sterilized YT media.
C. Each poured plate was allowed to dry for a minimum of 6 hours.
Source, cultivation, and preparation of bacterial samples:
A. The YT media was autoclaved and poured into sterile plates and
allowed to set and dry until no liquid was free on the
surface of the plates.
B. Strain S. aureus (S-1) received from the New York
Hospital (Queens County, NY)
(i)
25952 - Wild type
C. The bacteria were grown on YT media for 16 to 24 hours before
being harvested.
D. A confluent inoculum of S-1 was re-suspended in 3ml of sterile,
purified (17 to 18 MegOhm lab water until a 0.3%
Transmittance was
achieved at 375nm as indicated by spectrophotometry.
(i)
A standard 10:1 dilution series was performed using sterile,
purified lab water.
1/10, 1/100, 1/1,000, 1/10,000 and 1/1000,000
dilutions of bacterial suspension were prepared.
Control Preparations
A. The Negative Control consisted of substituting sterile, purified
lab water for product in the same volume as product.
Treatment of Bacteria with Colloidal Silver
A. 10ul of each undiluted colloidal silver was added to 90ul of each
dilution of the bacterial stains separately.
a. This
changed the effective concentrations of both the mixtures and
bacterial suspensions:
i. Silver products were diluted 10% by addition to the
bacterial cultures.
ii. The bacterial cultures were diluted to 90% of each of
their nominal dilutions.
The exposure times of each set of permutations were limited to four
(4) minutes and eight (8) minutes for each type fo treatment until
spotted onto the YT Plate.
Inoculation of test suspensions on YT Media Plates
A. One 10ul sample of each exposure was plated on YT plates.
B. Negative control plate (positive growth) - No product was added
to S-1 culture. Sterile, purified water replaced the
volume (10ul)
of product.
C. Plates were then placed in a 37 degree C. incubator overnight (18
hours).
D. The results were assessed according to the following criteria:
a. (+) represents strong, confluent solid spots of growth (1 point).
b. (1/2) represents some S. aureus growth, with some
confluence (1/2 point)
c. (1/4) represents a very few individual spots of growth. (1/4
point)
d. (-) represents no
S. aureus
growth (0 points).
Results
The negative control for S-1 grew out 4.5 spots. These samples had
purified lab water substituted for the fluid of the silver colloids.
The right most + or - character represents a 1/100,000 dilution
of the stock bacterial suspension; the left most + or - represents a
1/10 dilution. The more - characters from the right, the more potent
the antibacterial activity,
since about 10X more bacteria were
present in each successive spot inoculation (from right to left)
Spot Testing Results... |